PR domain containing 9 (Prdm9) is a gene specifying hotspots of meiotic recombination but in hybrids between two mouse subspeciesPrdm9controls failure of meiotic chromosome synapsis and hybrid male sterility.We have previously reported thatPrdm9-controlled asynapsis and meiotic arrest are conditioned by the inter-subspecific heterozygosity of the hybrid genome and we presumed that the insufficient number of properly repaired PRDM9-dependent DNA double-strand breaks (DSBs) causes asynapsis of chromosomes and meiotic arrest (Gregorovaet al.2018).We now extend the evidence for the lack of properly processed DSBs by improving meiotic chromosome synapsis with exogenous DSBs.A single injection of chemotherapeutic drug cisplatin increased frequency of RPA and DMC1 foci at the zygotene stage of sterile hybrids,enhanced homolog recognition and increased the proportion of spermatocytes with fully synapsed homologs at pachytene.The results bring a new evidence for a DSB-dependent mechanism of synapsis failure and infertility of intersubspecific hybrids.
- Jiri Forejt
- Jiri Forejt
- Barbora Valiskova
The funders had no role in study design,data collection and interpretation,or the decision to submit the work for publication.
Animal experimentation: The project was approved by the Animal Care and Use Committee of the Institute of Molecular Genetics AS CR,protocol No 141/2012.The principles of laboratory animal care,Czech Act No.246/1992 Sb.,compatible with EU Council Directive 86/609/EEC and Appendix of the Council of Europe Convention ETS,were observed.
- Patricia J Wittkopp,University of Michigan,United States
- Received: October 3,2018
- Accepted: December 27,2018
- Accepted Manuscript published:December 28,2018 (version 1)
© 2018,Wang et al.
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